esp3i (New England Biolabs)
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Structured Review
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esp3i
Esp3i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/esp3i/Esp3I/pmc13610606-37-11-12
Average 98 stars, based on 549 article reviews
Esp3i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/esp3i/Esp3I/pmc13610606-37-11-12
Average 98 stars, based on 549 article reviews
esp3i - by Bioz Stars,
2026-10
98/100 stars
Images
1) Product Images from "A Dual-Plasmid CRISPR/Cas9-Based Genome Editing System for Efficient Gene Knockout and Large DNA Integration in Cronobacter malonaticus"
Article Title: A Dual-Plasmid CRISPR/Cas9-Based Genome Editing System for Efficient Gene Knockout and Large DNA Integration in Cronobacter malonaticus
Journal: Pathogens
doi: 10.3390/pathogens15090990
Figure Legend Snippet: Plasmid map and cloning site distribution of the pAmpCRISPR/pCasCm system. ( a ) Plasmid map of pAmpCRISPR. tac promoter, the sgRNA expression promoter; MCS, multiple cloning site; Esp3I, the sgRNA sequence was cloned between two Esp3I restriction sites; Amp R , ampicillin resistance gene; oriT , origin of transfer; sacB , a sucrose counter-selectable marker. ( b ) Plasmid map of pCasCm. Expression of the λ-Red recombination system (Gam, Beta, and Exo) and Cas9 protein is driven by the L-arabinose-inducible araB promoter (P araB ); pSC101, a temperature-sensitive replicon; Tc R , tetracycline resistance gene. ( c ) Cloning site sequence of the pAmpCRISPR plasmid.
Techniques Used: Plasmid Preparation, Cloning, Expressing, Sequencing, Clone Assay, Marker
Related Articles
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At the end of the study, mice were humanely euthanized by cervical dislocation under deep isoflurane anesthesia, in accordance with the guidelines of the Animal Care and Use Committee of the National Institute of Infectious Diseases. mRNA synthesis and LNP formulation The complete sequence of the SARS-CoV-2 ancestral spike protein, incorporating two key mutations (K986P and V987P) to enhance structural stability, was codon optimized and cloned into a plasmid containing a T7 promoter, a 5′ UTR derived from human α-globin, a 3′ UTR from aminoterminal enhancer of split (AES) mRNA and mitochondrially encoded 12S rRNA (mtRNR1)37, and a AR TI CL E IN P RE SS poly-A tail designed for mRNA synthesis. mRNA was synthesized using the IVTpro T7 mRNA Synthesis Kit (Takara Bio, #6144) with a linearized plasmid digested by Article Title: A proteome-wide dependency map of protein interaction motifs. 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The annealed duplex was diluted 1:200 and cloned into Formulation:Article Title: A poorly reactogenic lipid nanoparticle-mRNA vaccine unveils an innate immune pathway for adverse reactions. Article Snippet: .. 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At the end of the study, mice were humanely euthanized by cervical dislocation under deep isoflurane anesthesia, in accordance with the guidelines of the Animal Care and Use Committee of the National Institute of Infectious Diseases. mRNA synthesis and LNP formulation The complete sequence of the SARS-CoV-2 ancestral spike protein, incorporating two key mutations (K986P and V987P) to enhance structural stability, was codon optimized and cloned into a plasmid containing a T7 promoter, a 5′ UTR derived from human α-globin, a 3′ UTR from aminoterminal enhancer of split (AES) mRNA and mitochondrially encoded 12S rRNA (mtRNR1)37, and a AR TI CL E IN P RE SS poly-A tail designed for mRNA synthesis. mRNA was synthesized using the IVTpro T7 mRNA Synthesis Kit (Takara Bio, #6144) with a linearized plasmid digested by Article Title: Integrative screening identifies functional variants and VNTRs underlying GWAS signals at the 5p15.33 multi-cancer susceptibility locus Article Snippet: Amplified products were cloned into the pXPR_050 vector (Addgene #96925) using the same emulsion PCR strategy described above for MPRA. ePCR products were purified with a QIAquick PCR Purification Kit (QIAGEN). .. The pXPR_050 backbone was digested with Article Title: A Dual-Plasmid CRISPR/Cas9-Based Genome Editing System for Efficient Gene Knockout and Large DNA Integration in Cronobacter malonaticus Article Snippet: .. The pAmpCRISPR plasmid was constructed as follows: fragments flanking the two Derivative Assay:Article Title: A poorly reactogenic lipid nanoparticle-mRNA vaccine unveils an innate immune pathway for adverse reactions. Article Snippet: .. At the end of the study, mice were humanely euthanized by cervical dislocation under deep isoflurane anesthesia, in accordance with the guidelines of the Animal Care and Use Committee of the National Institute of Infectious Diseases. mRNA synthesis and LNP formulation The complete sequence of the SARS-CoV-2 ancestral spike protein, incorporating two key mutations (K986P and V987P) to enhance structural stability, was codon optimized and cloned into a plasmid containing a T7 promoter, a 5′ UTR derived from human α-globin, a 3′ UTR from aminoterminal enhancer of split (AES) mRNA and mitochondrially encoded 12S rRNA (mtRNR1)37, and a AR TI CL E IN P RE SS poly-A tail designed for mRNA synthesis. mRNA was synthesized using the IVTpro T7 mRNA Synthesis Kit (Takara Bio, #6144) with a linearized plasmid digested by Synthesized:Article Title: A poorly reactogenic lipid nanoparticle-mRNA vaccine unveils an innate immune pathway for adverse reactions. Article Snippet: .. 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